Rapid, Flexible, and Scalable Sequencing-as-a-Service with Secure Cloud Delivery

High-Capacity Sequencing for Ready-to-Run Libraries

Accelerating clinical and research genomics through flexible, high-quality sequencing services using the Illumina NovaSeq X Plus — offering seamless cloud-based data delivery and unmatched scalability.

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Overview

Karkinos offers sequencing-as-a-service offerings for diagnostic laboratories, research organizations as well as service providers enabling convenient and flexible data throughputs, rapid turnaround times and high quality data. With a rich experience working with clinical and research samples over the years, and being one of the first and largest installation bases for NovaSeq X plus in India we offer the best of its class services to our customers. 

This service is designed for customers who would be preparing sequencing-ready libraries themselves and would like to access sequencing services of the pooled libraries. Library preparation service is not part of these offerings. We offer sequencing services on Illumina NovaSeq X Plus.

Sequencing Options

Karkinos provides an array of sequencing service options to fit in your specific needs 

Sequencer Options

Minimum Order Size

Turnaround Time

Flexible

Illumina NovaSeq X Plus

Flexible (starting from 25 Gigabases)

14 days

Gold

Illumina NovaSeq X Plus

Full Lane

7 days

Platinum

Illumina NovaSeq X Plus

Full Flow cell

5 days

All turnaround times are calculated from the date of receipt of samples / accession to the date of run completion.

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All turnaround times are calculated from the date of receipt of samples / accession to the date of run completion.

Data Delivery

The Data would be delivered on customer-specified and customer-owned cloud services including Microsoft Azure, Google Cloud or Amazon Web Services as BCL files (for Gold and Platinum) or demultiplexed FASTQ files. 

The Process

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Step 1

Tell us more about your sequencing requirements
and your proposed timelines
You could email us at lab@karkinos.in

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Step 2

Our project manager would reach out to you to understand your requirements in detail, and also get back with more details and work out a plan to schedule your samples and sequencing once the requisite information is collected.

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Step 3

Ship your samples to the lab. We will update you once the samples are received and scheduled for sequencing.

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Step 4

We will inform you once the samples are sequenced, along with the QC parameters and deliver the data in your cloud platform, ready for further analysis at your end.

Input Requirements 

The library volume and concentration (qPCR or Qubit) must meet specifications as below. The libraries must be normalized and pooled by the customer prior to submission along with the index sequence information in the specified template for libraries that would require demultiplexing to FASTQ files for delivery.

Libraries on Illumina NovaSeq X Plus should have a minimum library volume of 50µL and a minimum library concentration of 2nM. 
The libraries should be accompanied by Tapestation profiles. If not available, the profiles may be generated on our end at an additional cost.

Minimum Information Required

While requesting for the sequencing services, customers are requested to provide the following specifications in a specified template for your prepared library:

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Library size (bp)

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Read Structure

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Read length (should agree with the number of cycles)

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Paired end (PE) or Single end (SE)

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Index Structure

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Single or dual indices

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Index length (maximum of 10bp)

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Sequence details for i7/p7 and i5/p5 indexes (forward and reverse sequence)

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Loading concentration (in pM)

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Expected total fragment size (insert + adapters)

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PhiX spike-in percentage (Optional) 

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Demultiplexing key (Optional) 

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If applicable, custom primers/sequences must also be provided 

  • Library size (bp)
  • Read length (should agree with the number of cycles)
  • Index Structure
  • Index length (maximum of 10bp)
  • Loading concentration (in pM)
  • PhiX spike-in percentage (Optional)
  • If applicable, custom primers/sequences must also be provided
  • Read Structure
  • Paired end (PE) or Single end (SE)
  • Single or dual indices
  • Sequence details for i7/p7 and i5/p5 indexes (forward and reverse sequence)
  • Expected total fragment size (insert + adapters)
  • Demultiplexing key (Optional)